What is darkfield microscopy and how is it different to bright field microscopy? Let me tell you a thing or two about darkfield microscopy and why it is important. Darkfield microscopy entirely relies on a different illumination system rather than illuminating the sample. This is a type of a specialized illumination technique that capitalizes on oblique illumination to enhance contrast in specimens that are not imaged well under normal bright field illumination condition. On the other hand a darkfield illumination is a method by which a specimen (transparent or semi transparent) is seen as a bright object against a dark background. Using a darkfield microscope in a specimen the condenser forms a hollow cone of light and dark in the center. That is when the tip of the cone strikes the specimen, who therefore scatters light against a dark background; this will make transparent detail more visible than in a bright field microscope. This type of effect can compare to the glittery appearance of dust particles in a dark room illuminated by strong shafts of light coming in through a side window. The dust particles are very miniscule but are easily seen when they scatter the light rays, and that is the working principle of darkfield microscopy. This explains how the image of low contrast material is created; an object will be seen against a dark background if it scatters light, which is captured with the proper device such as an objective lens. We have prepared all the juicy details for you to discover as you flip on the pages of this website.